Vochysia rufa and glibenclamide promote effects on oxidative damage and in proteins implicated in vesicular trafficking in diabetic rat brain

Many Vochysiaceae species are widely used in folk medicine to treat some diseases. Vochysia rufa, popularly known as “quina-doce”, has been type 1 and 2 diabetes mellitus the state of Minas Gerais, Brazil. Although antidiabetic antioxidant effects phytochemical profile rufa have already elucidated, further studies needed on this treatment specific tissues, such brain. This study investigated effect aqueous extract diabetic rat brains for purpose, oxidative stress markers expression/localization proteins implicated vesicular trafficking were evaluated. Thirty-two rats randomized into four groups (non-diabetic, non-treated, treated 43 days with glibenclamide - 6 mg/kg or 500 mg/kg). The was administered by gavage days. Analyses conducted enzymes concentration activity protein levels localization myosin-Va, CaMKII, synapsin, SNAP-25 GLUT4 also analyzed. decreased superoxide dismutase, glutathione peroxidase, reduced glutathione, total sulfhydryl lipid peroxidation increased S-transferase levels. Additionally, expression CaMKII which confirmed immunolocalization. reduces protecting brain from damage caused hyperglycemia.